Mini beadbeater plus

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Leaves were grated in Mini-Beadbeater tissue disruptor and processed with the use of the Genomic Mini AX Plant SPIN kit (A&A Biotechnology) according to the manufacturer’s protocol. Figure 1: Geographic distribution of the analyzed populations of Pulsatilla patens . Nov 28, 2019 · Cells were then lysed in 100 μl sample buffer (65 mM Tris, pH 6.8, 3% SDS, 10% glycerol, 10% 2-mercaptoethanol, 50 mM sodium fluoride, 50 mM β-gylcerophos­phate, 1 mM sodium orthovanadate, complete EDTA-free protease inhibitor tablet [Pierce]) in a Mini-beadbeater-16 for 2 min. Blots were probed with anti-FLAG M2 (Sigma), anti-GST (Covance ... Mar 01, 2016 · disrupted with a BeadBeater (Biospec Products, Bartlesville, OK, USA). Cell debris was removed by centrifugation at 12,000 g for 10 min. A buffer exchange to 50 mM sodium phosphate buffer pH 8.3, 300 mM NaCl was done with 2 mL Zeba Desalt Spin Columns. Total protein concentration of the cell lysate was determined with the Coomassie Plus Assay ... The Mini-Beadbeater-Plus™ is an upgraded version of the venerable Mini-BeadBeater-1. Improvements include a programmable timer, a powerful brushless motor and 115/230 VAC compatibility. The high-energy bead-mill rapidly and completely disrupts microorganisms and plant and animal tissue. Brand Name: BioSpec. LEARN MORE Jul 10, 2013 · For DNA extraction, 50 mg of the frozen fecal sample was dissolved in 1 ml of extraction buffer (50 mM Tris (pH 7.4), 100 mM EDTA (pH 8.0), 400 mM NaCl, 0.5% SDS) containing 20 μL proteinase K (20 mg/ml). 500 μL of a slurry of 0.1 mm-diameter zirconia/silica beads (BioSpec Products) were added into the extraction tubes and a Mini-Beadbeater ... (A and B) Serial dilutions of the targeted microorganism (TMO) plus a fixed amount of internal control (IC) genomic DNA were processed in triplicate using an OmniLyse device for 1.5 min or a BioSpec Mini-BeadBeater/Mini-BeadBeater-8 Article Snippet: The Mini-BeadBeater and the Mini-BeadBeater-8 were purchased from BioSpec (Bartlesville, OK).

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Nov 19, 2019 · DNA was isolated from the mycelia using the DNA isolation from Plant kit and protocol (Nexttec GmbH). This included homogenization with 300 μl acid washed 0.1 mm Zirconium beads (Biospec Products) and 2 × 1 min bead-beating with cooling on ice in between (Mini-Beadbeater-96). The supernatant was directly used as template for PCR.
Cells were disrupted by shaking in a Mini-Beadbeater (Biospec Products, Bartlesville, OK) in the presence of equivalent amounts of acid-washed glass beads (120– 200 lm and 425–600 lm in diameter; Sigma-Aldrich, St. Louis, MO) and vortexed briefly. The samples were treated in the beadbeater for 30 s at 5000 r.p.m. DNA
(A and B) Serial dilutions of the targeted microorganism (TMO) plus a fixed amount of internal control (IC) genomic DNA were processed in triplicate using an OmniLyse device for 1.5 min or a BioSpec Mini-BeadBeater/Mini-BeadBeater-8 Article Snippet: The Mini-BeadBeater and the Mini-BeadBeater-8 were purchased from BioSpec (Bartlesville, OK).
Jan 10, 2013 · Genomic DNA Isolation From Different Biological Materials 7 3. Using a mini spatula, collect and resuspend the cells in 567 L TE buffer, add 3 L of 20 mg/mL proteinase K and 30 L of 10% SDS, and incubate for 30 min at 37°C (see Note 2). 4. Add 100 L of 5 M NaCl. Mix thoroughly (see Note 3). 5.
微珠研磨器(均質機) 廠牌: Biospec 型號: Mini-Beadbeater-16 詳規範 . ... Bioruptor Plus,詳規範 . 1/ST. D-M40UF3. 1. 2020/12/12. 2020/12/22 23:59:59.
Aug 09, 2011 · Samples were mechanically disrupted in a Mini-beadbeater-8TM cell disrupter (BioSpec Products, Inc., Bartlesville, OK) for 10 min. After disruption, samples were incubated on ice for 5 min and the beads were allowed to settle out of the lysis mixture. Samples underwent centrifugation (in an Eppendorf centrifuge at 2100 rcf 1 min).
77 DNA was extracted from cyst suspensions using the QIAamp DNA mini kit (Qiagen, 78 Hilden, Germany) after an initial disruption of the purified cysts with a Mini-BeadBeater 79 (Biospec Products Inc. , USA) (Lebbad et al., 2008).
Instruction manuals,user guides, operating instructions owners handbooks available and wanted for every category of product. Computers, printers microwave oven washing machine software book rare collectable hifi answerphone fax machine camcorder cooker fridge modem photocopier radio television video walkman scanner speaker camera great britain uk etc. United kingdom site.owners handbooks books ...
Whole insect specimens were homogenized for 1.5 min using a Mini-Beadbeater-16 (BioSpec), in 1.5 mL tubes containing one 3 mm glass bead and 80 μl of homogenization buffer (10 mM Tris, 1 mM EDTA, 50 mM NaCl [pH 8.2]), and were then centrifuged at 4°C for 30s at 13,000 rpm . The samples were then incubated at 97°C for 10 min, placed on ice ...
Approximately 30 mg of tissue was added to a chilled BioPulverizer H tube (Bio101, Solon, OH). Lysis buffer from the Qiagen RNeasy Mini kit (Qiagen, Valencia, CA) was added, and the tissue was homogenized for 20 seconds in a Mini-Beadbeater (BioSpec Products, Bartlesville, OK). Tubes were spun briefly to pellet the garnet mixture and reduce foam.
Jul 10, 2013 · For DNA extraction, 50 mg of the frozen fecal sample was dissolved in 1 ml of extraction buffer (50 mM Tris (pH 7.4), 100 mM EDTA (pH 8.0), 400 mM NaCl, 0.5% SDS) containing 20 μL proteinase K (20 mg/ml). 500 μL of a slurry of 0.1 mm-diameter zirconia/silica beads (BioSpec Products) were added into the extraction tubes and a Mini-Beadbeater ...
All sample fractions were first lyophilized and then homogenized with 10 -mm stainless-steel beads for 2 min using a Mini-Beadbeater-96 (BioSpec Products, Bartlesville, OK). A mixture of deuterium-labeled internal standards (IS) was added to 50 mg of each dried fraction, together with 3 mL of extraction solvent (IPA:H 2 O:AcOH, 80:19:1).
Tissue and fecal homogenization was performed by mechanical disruption for 2 × 60 s with 2.3 mm zirconia/silica diameter beads (Biospec) and the Mini‐Beadbeater‐16 (Biospec). For PERV screening in transplant recipients, 200 μl of human WBC (prepared identically to that described for porcine WBC) and 200 μl of blood plasma were taken at 1 ...
Oct 13, 2019 · This mix was autoclaved at 134 °C for 1 h. One mL of the autoclaved sample was smashed using a mini-beadbeater (BioSpec Products Inc., USA) to fully release the starch from the cells, and 0.5 mL of the smashed sample was mixed with 0.5 mL of the sodium phosphate buffer then subjected to the double digestions aforementioned.
Mini-Beadbeater-1. The Mini-Beadbeater-1 violently agitates a sealed microcentrifuge vial containing cells or tissue, extraction solution and tiny glass, zirconia or steel beads.
In laboratories that lack access to a Mini-Beadbeater-24 (BioSpec Products, 16 Bartlesville, OK), the use of a Vortex Adapter for Vortex-Genie® 2 (bioMérieux SA, P/N 270677
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4′-O-methylhonokiol (MH) is a natural product showing anti-inflammatory, anti-osteoclastogenic, and neuroprotective effects. MH was reported to modulate cannabinoid CB2 receptors as an inverse agonist for cAMP production and an agonist for intracellular [Ca2+]. It was recently shown that MH inhibits cAMP formation via CB2 receptors. In this study, the exact modulation of MH on CB2 receptor ...
Briefly, 250 µg of frozen flavedo were extracted with 1500 µL of ethanol in a Mini Beadbeater 8 Cell Disruptor (Biospec Products, Inc.). The extract was centrifuged at 13000 × g for 5 min at 4 °C and two supernatant aliquots were used to determine the total flavonoid content.

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Jan 27, 2009 · About 4 volumes of extraction buffer containing 100 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, pH 7.2), 100 mM KCl, and 2 mM EDTA, as well as 1 mg/ml protease inhibitors, was added to each liver sample to yield an approximately 28% (w/v) homogenate. The liver tissues were homogenized with Mini Beadbeater.
May 30, 2012 · For sale from HiTechTrader - Biospec Mini Bead Beater Model 3110BX, Power 115 V, 60 Hz, 0.62 A. Vairable digital speed and time controls. Speed from 2,500-5,000rpm and timer can be set from 10-100 ...
Mini BeadBeater Plus. 11079110sc by Biospec. Price: $45.00 / EA. Silicon Carbide Particles 1.0mm. 110803-15SS by Biospec. Price: $242.00 / EA. 15ml Small Chamber S.S ...
50 mM PIPES buffer plus 0.3 M NaCl (pH 7) to account for possible differences in protein interaction due to buffer choice or salt concentration. Cell cultures were homogenized using a Mini-Beadbeater 8-Cell Disrupter (Biospec Products, Inc.) and 0.1-mm zirconia/silica beads for 60 s to release the cytoplasmic con-tents.
BioSpec mini beadbeater 8 Mini Beadbeater 8, supplied by BioSpec, used in various techniques. Bioz Stars score: 94/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
For much smaller prep- arations a Mini-Beadbeater-8 cell disrupter (BioSpec Products) ac- commodates 2-ml microcentrifuge tubes. Growth of Cells for Protein Purification A high cell density and a high ratio of glass beads to cells in the lysis step greatly enhance the efficiency of breakage.
The Mini-BeadBeater-Plus is also used for dry- or cryo-grinding. Heavier zirconia or steel beads and special breakage-resistant microvials are used. Hard samples such as hair, bone, teeth, seeds, and minerals are powdered in 5 – 30 seconds.
Analytical Abstracts is a current awareness and information retrieval service for analytical chemistry, published by the Royal Society of Chemistry in Cambridge, United Kingdom. It was first published in the mid-1950s by the Society for Analytical
The Mini-Beadbeater-16 is a high-energy cell disrupter designed to handle four to sixteen samples at a time. The Mini-BeadBeater-16 disrupts microbial cells and plant and animal tissue by violently agitating four to sixteen 2 ml screw-cap microvials containing small glass, ceramic or steel beads and disruption buffer.
The Mini-Beadbeater-Plus™ is an upgraded version of the venerable Mini-BeadBeater-1. Improvements include a programmable timer, a powerful brushless motor and 115/230 VAC compatibility. The high-energy bead-mill rapidly and co...
After one week, the leaves were cut, transferred to a 2 mL Eppendorf tube containing three stainless steel beads (2.3 mm in diameter), frozen in liquid nitrogen and triturated in a Mini-Beadbeater TM (Biospec Products) for 1 min. Total DNA was extracted using a CTAB-based protocol (Doyle and Doyle, 1987) and quantified on 0.8% agarose gels.
Cerebellums were homogenized in a Mini-BeadBeater (Biospec Products, Inc., Bartlesville, OK). Brain samples and calibration standards in brain homogenate were prepared for liquid chromatography-tandem mass spectroscopy (LC-MS/MS) by precipitating proteins with acetonitrile and vacuum filtration in the presence of an internal standard (EVP ...
The bacterial stringent stress response, mediated by the signaling molecule guanosine tetraphosphate, ppGpp, has recently gained attention as being important during normal cellular growth and as a potential new therapeutic target, which warrants detailed mechanistic understanding. Here, we used intracellular protein tracking in Pseudomonas aeruginosa PAO1, which indicated that RelA was bound ...
Nov 19, 2019 · DNA was isolated from the mycelia using the DNA isolation from Plant kit and protocol (Nexttec GmbH). This included homogenization with 300 μl acid washed 0.1 mm Zirconium beads (Biospec Products) and 2 × 1 min bead-beating with cooling on ice in between (Mini-Beadbeater-96). The supernatant was directly used as template for PCR.
Nov 28, 2019 · Cells were then lysed in 100 μl sample buffer (65 mM Tris, pH 6.8, 3% SDS, 10% glycerol, 10% 2-mercaptoethanol, 50 mM sodium fluoride, 50 mM β-gylcerophos­phate, 1 mM sodium orthovanadate, complete EDTA-free protease inhibitor tablet [Pierce]) in a Mini-beadbeater-16 for 2 min. Blots were probed with anti-FLAG M2 (Sigma), anti-GST (Covance ...



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